Decoction for Recovery and Relieving Pain(DRRP) is a key project of Ministry of Education to do the research on the DRRP. The article is about research on the identification methods of the paregoric constituent of DRRP, divided into three parts: identification of the source of the paregoric composition; study on the compatible relationship of the herb groups; exploration of the fuzziness quantitative method of the herb slices. The project established the basis of the development of DRRP and explored beneficially the method to institute the quality criteria of the herb slices and compound traditional Chinese medicine.  1. Identification of the source of the paregoric composition. Objective: ascertain the source of the paregoric constituent of DRRP.Methods: divide the whole prescription into six effectiveness groups: replenishing qi and enriching the blood, promoting blood circulation by removing stasis, dredging collaterals to relieve pain and so on. Do the prescription-breaking experiment according to the L16(215) orthogonal experiment design table, with the average writhing frequency of the acetic acid writhing experiment as statistical index. Results: P(C)<0.01, P(B,D and C×D)<0.05, P(A and B×C)<0.1. The results show that the factors of B, C, D and C×D has conspicuous effective on the writhing frequency and the factors of A and B×C has relatively conspicuous effective on that.Conclusions: The result of screening is A1B1C1D1E2F2 and the paregoric prescription is the combination of eight Chinese medicine Radix Astragali, Radix Angelica sinensis, Senmen pruni persicae, Flos Carthami and another four chinese medicine(need to be a secret) and ascertain the source of the paregoric constituent.  2. Study on the compatible relationship of the herb group.Objective: Investigate the interaction of the contents (mainly polyose, saponin and astragalosideⅣ) of the Chinese medicine in the herb group.Methods: According to 22 and 23 factorial experiment design, phenol-sulphuric acid method, vanillin-perchloric acid method and HPLC were used to study the effects of Radix Angelica sinensis, Senmen pruni persicae, Flos Carthami to Radix Astragali.Results: Radix Angelica sinensis and two other Chinese medicine could make the content of polyose higer,the content of astragalosideⅣlower;but could not influence the content of. Saponin in the extract. Conclusions: The contents of polyose and astragalosideⅣof Radix Astragali were significantly influenced by the compatibility of the three Chinese medicine in the water extract in the herb group..  3. Exploration of the fuzziness quantitative method of the herb slices. Objective: Radix Astragali as a whole, explore the feasibility of the fuzziness quantitative method of the standard herb slices established to determine the relative quantity of it in the herb groups. Methods: HPLC was used for determination. The chromatographic condition: HypersilODS2(4.6mm×250mm,5μm) as Analytica Column; H2O-ACN as mobile phase gradient eluting; wave length for determination 254nm; room temperature(18～22℃) as the column temperature; flow rate 1.0mL/min; aethylparabenum as the internal standard. Draw the HPLC characteristic chromatogram of three batches of Radix Astragali (A,B,C) and the herb groups, select four common characteristic peaks (1,2,3,4) and contrast the proportion relationship the common characteristic peaks of three batches of the herb slices and the herb group. Determine the relative quantity of the Radix Astragali herb slice A, C and the Radix Astragali of three batches of herb group compared with B as a standard by mean of internal standard contrast method.  Results: The relative quantity of herb slice A,C were 100.7%±1.15%, 79.34%±0.21%; The relative quantity of Radix Astragali in the herb group A,B,Cwere 26.84%±0.22% , 21.34%±0.10% and 20.94%±0.32%. The stability test showed that the sample had favorable stability in 48h. Conclusions: The method is basically feasible to determine the relative quantity after selecting the unknown characteristic components of Radix Astragali under the definite HPLC experimental condition.