Objective In order to explore ideal tissue for determination of the late time of death, and establish linear regression equation between mRNA degradation and postmortem intervals, the rate of mRNA degradation from housekeeping gene GAPDH andβ-actin investigated. One step RT-PCR technique and Real time RT-PCR technique are applied in evaluating the correlation between rate of mRNA degradation in SD rats and postmortem intervals, especially in advanced stage PMI.


  Methods (1) A total of 28 SD rats were gro...