ObjectiveEpigenetics is the study of gene expression may be subject to genetic change and the genomic DNA sequence is not generated.At present,the study including:DNA methylation,histone code and chromatin remodeling and regulation of non-coding RNA.The abnormal interaction in their system can lead to the gene abnormal expression and lead to epigenetic-related diseases including the tumor.The occurrence of tumor is due to abnormal cell proliferation,in the past,the vast majority of studies focus on proto-oncogene and anti-oncogene witch encoded protein.However,with the progress of the study we found that some tumors less or no gene mutation,as well as miRNA family strong accommodated in post-transcriptional level,so that an increasing number of researchers to shift the emphasis to the relationship between miRNA and tumor.Dicer is an enzyme essential for the processing of miRNA.When the Dicer gene expression abnormal may lead to the miRNA expression abnormal.Those make proto-oncogene expression increase or anti-oncogene expression decrease and then cause the cell or tissue abnormal proliferation,resulting in the occurrence of tumor.In order to investigate the function of Dicer gene overexpression in tumor,we designed this experiment,human embryonic lung cells(HELF) were stabily transfected of pcDNA3.1-Dircer plasmid,by detecting HELF proliferation and migration to analyze the function of Dicer gene in the tumor.MethodsHELF cells were stabily transfected of pcDNA3.1-Dicer plasmid and pcDNA3.1 vector as control by lipofectamine.1:7 generated at the time of transfection 48 hours.G418 selected then picking single clone.Initial screen Dicer gene DNA horizontal conform by PCR.The transfection effects of Dicer gene on the mRNA levels were examined by reverse transcription PCR(RT-PCR),and the transfection effects of Dicer gene on the protein levels were examined by Western-blot.Cell proliferation rate and viability were measured by MTT.Reconstituted basement membrane invasion assay was utilized to evaluate the cell invasive.Analyze the function of Dicer gene in the tumor.ResultpcDNA3.1-Dicer plasmid and pcDNA3.1 vector were transfected into HELF by lipofectamine.G418 selected then picking single clone.Dicer mRNA and protein expression were obvious upregulated in pcDNA3.1-Dicer plasmid transfected HELF cells than control vector transfected cells,which illuminates cell transfection of pcDNA3.1-Dicer successfully.MTT incorporation rate were increased significantly was apparent in pcDNA3.1-Dicer plasmid transfectedHELF cells.Transwell migration assay results showed HELF cells migration of pcDNA3.1-Dicer plasmid transfection was increased significantly compared with vector pcDNA3.1 transfected HELF cells.ConclusionStabily transfected HELF of pcDNA3.1-Dicer plasmid can lead to upregulate Dicer mRNA and protein expression obviously,which showed transfection HELF of pcDNA3.1-Dicer successfully.MTT incorporation rate were increased significantly was apparent in pcDNA3.1-Dicer plasmid transfected HELF cells and Transwell migration assay results showed HELF cells migration of pcDNA3.1-Dicer plasmid transfection were increased significantly compared with vector pcDNA3.1 transfected HELF cells.