To explore inducement resistance of S.japonicum with administration of drug-pressure ,we estimate praziquantel(PZQ) sub-curative drug dose(ED_(50)) and continuously use PZQ ED_(50) to administrate mice that were infected by S.japonicum for 6 weeks. Evaluate biology affect of PZQ-resistant of S.japonicum with different inducement ways and observe PZQ-resiestant or PZQ-susceptibility, then obtain the best way of inducement. The results demonstrate that ED_(50) of PZQ is 20.89mg/kg.Data suggest that S.japonicum are lower susceptibility to PZQ with the more continuouse inducement days. Especially, the data show that administration of praziquantel after consecutive 30 days,the least fatal dose of S.japonicum in vitro have experienced a significant increase, from 4.4μg/ml(normal worm) up to 36μg/ml . The findings provides experimental basis to farther explore mechanism of PZQ-resiestant.We also investigate anti-schistosomal effect of five kinds of Diazepam derivatives B3、B7、B8、B26、B30 that have some effects on S.mansoni. The results showed that the activity of B3 and B30 on schistosomes was obvious in vitro (P<0.01).To investigate the anti-schistosomal mechanism of Diazepam derivatives,cytochalasin D(CyD) and calcium channel blockers were used to against the effects of B3 and B30.The results also showed that CyD and calcium channel blockers inhibited the activity of B3 and B30 against S.japonicum.. It was found that S.japonicum have lower susceptibility to PZQ , and the low susceptibility to B3 and B30 after inducement resistance of S.japonicum with administration of PZQ-pressure. Diazepam derivatives presents similarities with PZQ with regard to its anti-schistosomal mode of action, since both agents may be involved in the calcium channels of schistosome.