Objective: To study the effcets of diffent concentration and action time of Triamcinolone Acetonide on the proliferation and apoptosis of hypertrophic scar fibroblasts in vitro; to investigate the mechanism of Triamcinolone Acetonide in treatment of hypertropic scar. And to find out the optimal concentration of Triamcinolone Acetonide for clinical treatment of hypertropic scar.  Methods:  1. Collect tissue of hypertrophic scars from the hospital.  2. Culture and purify the HFB in vitro in the tissue cultured system.  3. The cultured HFB were identified by the morphological and immune-histochemistrical methods.  4. Different concentration of Triamcinolone Acetonide were administered to the cultured HFB for 24hours, 48hours, and 72hours.Set the non- Triamcinolone Acetonide as negative contrast.  5. The proliferative activity of HFB were detected by MTT method.  6. The electric-microscope and the Tunel method were used to detect the apoptosis of HFB.  7. Collect and analyze data.  Result:  1. The cultured cell were proved of HFB by the morphological evidence and the immune-histochemistrical evidence.  2. The MTT test showed that : The inhibiting rate of HFB was increasedobviously with the concentration of Triamcinolone Acetonide(P<0.01). The inhibiting rate was in proportion to the administered time and theconcentration of Triamcinolone Acetonide. And the IC50 in 24h,48h,72h are0.42g/L,0.21g/L and 0.10g/L.The IC95 in 24h,48h,72h are