DNA delivery, especially via nonviral carrier system, has become a powerful and popular research tool for gene therapy. It has also been pivotal in developing new approaches for treating diseases such as cancer. A major requirement for DNA delivery system is the efficient transport of DNA through cell membrane. The mechanistic pathway for DNA transport includes the collapse of extended DNA chains into compact, orderly particles containing only one or a few molecules. This process, known as DNA condensation, has received considerable attention in recent years due to its biological importance in DNA packaging in virus heads as well as in the development of gene delivery vehicles. DNA condensation improves both chemical stability and physical properties of DNA, and the positive potential of DNA condensates improves the transport efficiency. This study aimed to deliver antisense oligonucleotides (ASON, 20bp) to tumor cells. ASON complementary to human telomerase reverse transcriptase mRNA (hTERT mRNA ) can hybridize and specifically inhibit the expression of hTERT, thus offering the possibility of specific, rational, genetic-based therapy for tumor.This study consisted of three parts. In the first part, ASON condensation by poly-L-lysine (PLL) was investigated. In the second part , NGR peptide consisting of 16 lysines and one NGR motif was used to modify the condensates of ASON-PLL condensates. The NGR motif is an aminopeptidease N (CD13) ligand that targets