Following two pieces of opinion on hospitals innovation have been issued for enforcementsince 2000,which devided medical organizations into two parts-profitable and non-profitableorganization,it was the beginning of innovation for medical system.All hospitals should paymore attention to customers'demand ,operate in a fair and ordered circumstance to reach marketshare.This article's research point is about the non-profit hospital, through summarizing somerealizations on non-profit organization and the application of marketing theory,to help peoplerealize that it is very important for non-profit hospital to use marketing theory. It suggestsnon-profit hospital to take balance well betwwen non-profit and payoff.The marketing target ofnon-profit hospital is to serve sufferer better.And then try to put forward some Non-profitHospital'Marketing Strategies through analyzing circumstance around non-profit hospital ,takingmarket segmentation and market positioning.